Immunology Core Services

The CFAR Immunology Core provides sophisticated immunology assays and expert consultation to the HIV research community, develops specialized immunology research tools, and initiates and stimulates innovative research projects that address emerging questions in HIV research. Contact Director Jeffrey Milush to discuss your project.

Laboratory Services

Immunology Core services are provided in response to the needs of CFAR investigators and in response to the CFAR scientific themes. Current services include:

  • Isolation of PBMC for staining, cell sorting, or viable cryopreservation
  • Isolation of mucosal mononuclear cells (MMC) from mucosal tissue biopsies
  • Immunomagnetic bead isolation of untouched cell populations for functional studies
  • Immunophenotyping of whole blood, PBMC, MMC and cerebrospinal fluid for: NK, B and T cells percentages, and their subsets, including maturation, trafficking, senescence, activation, in vivo proliferation and chemokine receptor expression
  • Flow cytometric functional assays, including NK cell function, antigen-specific CD4+ and CD8+ T cell cytokine expression, degranulation and proliferation
  • Evaluation of T cell signaling by phosflow using monoclonal antibodies specific for phosphorylated protein

Training and Support

Flow Cytometry Training Course

  • A three-hour lecture and lab course designed to cover the basics of flow cytometry including:
    • Fluorescence emission and detection
    • Fluidics
    • Operation
    • Panel design
    • Controls
  • Click here to reserve a spot for the next available course.

Flow Clinic (every other Monday 11-12)

  • A free, drop-in, one hour forum where users can bring data and discuss any issues or pose questions that they have about flow cytometry.
  • Contact us to find out about the next meeting