Flow-cytometry based immunophenotyping of whole blood, PBMC, MMC and cerebrospinal fluid

o Including but not limited to NK, B and T cell percentages and their subsets (including maturation, trafficking, senescence, activation, in-vivo proliferation and chemokine receptor expression)

· Flow cytometric functional assays

o Including but not limited to NK cell function, antigen-specific CD4+ and CD8+ T cell expression, degranulation and proliferation

o Evaluation of T signaling by phosflow using monoclonal antibodies specific for phosphorylated protein